RNA: packaged and protected by VLPs

Isolation and identification of Bacillus cereus of dry bulk and packaged vegetables by culture and PCR

Background: Bacillus cereus is an endemic, Gram-positive, rod-shaped, motile, beta hemolytic bacterium. Some strains are harmful to humans and cause foodborne illness and it's spore is resistant against difficult condition In recent years life style and feeding habits have changed and an increase in the use of ready to eat product has raised concern about quality and health assessment. Dehydrat...

Radial distribution of RNA genomes packaged inside spherical viruses.

The problem of RNA genomes packaged inside spherical viruses is studied. The RNA-capsid attraction is assumed to be nonspecific and occurs at the inner capsid surface only. For weak attraction, RNA concentration is maximum at the center of the capsid to maximize their configurational entropy. For stronger attraction, RNA concentration peaks near the capsid surface. In the latter case, the compe...

Developing Virus-Like Particles (VLPs) and Heterologous VLPs Vaccines for Epizootic Hemorrhagic Disease Virus (EHDV) Serotypes

Enhancement of RNA Interference Effect in P19 EC Cells by an RNA-dependent RNA Polymerase

Background: RNA interference (RNAi) is a phenomenon uses double-stranded RNA (dsRNA) to specifically inhibit gene expression. The non-specific silencing caused by interferon response to dsRNA in mammalian cells limits the potential of utilizing RNAi to study gene function. Duplexes of 21-nucleotide short interfering dsRNA (siRNA) inhibit gene expression by RNAi. In some organisms, siRNA can als...

Defective retrovirus-like 30S RNA species of rat and mouse cells are infectious if packaged by type C helper virus.

RNA species with properties of defective retrovirus-like 30S RNA genomes have previously been detected in both rats and mice and in some rat and mouse retroviruses. Using cell lines which express high levels of this retrovirus-like RNA, we formed pseudotypes of the 30S RNAs with helper-independent type C viruses. A pseudotype virus complex containing a mouse 30S subunit was transmitted to rat c...